mouse anti human icam (R&D Systems)
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Mouse Anti Human Icam, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 110 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+human+icam+1/Human+ICAM-1%2FCD54+Antibody/pm41944190-237-44-47
Average 93 stars, based on 110 article reviews
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Immunostaining:Article Title: Inflamed lymphatic endothelium suppresses dendritic cell maturation and function via Mac–/ICAM-1-dependent mechanism Article Snippet: Mouse anti-human podoplanin used for FACS was from AngioBio (DelMar, CA). .. Abs used for immunostaining were: rat anti-mouse ICAM-1 (SouthernBiotech, Birmingham, AL), Incubation:Article Title: Mastic Oil Inhibits the Metastatic Phenotype of Mouse Lung Adenocarcinoma Cells Article Snippet: .. EC were treated with medium containing various concentrations of mastic oil (0.01–0.04% v/v), POH (0.5–1.0 mM) or DMSO vehicle (0.1%) for 2 h. Subsequently, tumor necrosis factor-alpha (TNF-α, 10 ng/mL) or PBS was added and cultures were further incubated for 4 h. Cells were then solubilized and lysates were resolved by 10% SDS-PAGE as described previously [ ] using Article Title: VEGF suppresses T-lymphocyte infiltration in the tumor microenvironment through inhibition of NF-κB-induced endothelial activation. Article Snippet: Antiangiogenic treatment targeting the vascular endothelial growth factor (VEGF) signaling pathway is in clinical use, but its effect on vascular function and the tumor microenvironment is poorly understood.. Here, we investigate cross-talk between VEGF and proinflammatory TNF-a signaling in endothelial cells and its impact on leukocyte recruitment.We found that cotreatment with VEGF decreased TNF-ainduced Jurkat cell adhesion to human microvascular endothelial cells by 40%.. This was associated with inhibition of TNF-a-mediated regulation of 86 genes, including 2 T-lymphocyte-attracting chemokines, CXCL10 and CXCL11 [TNF-a concentration 1 ng/ml; 50% inhibition/inhibitory concentration (IC50) VEGF, 3 ng/ml]. SDS Page:Article Title: Mastic Oil Inhibits the Metastatic Phenotype of Mouse Lung Adenocarcinoma Cells Article Snippet: .. EC were treated with medium containing various concentrations of mastic oil (0.01–0.04% v/v), POH (0.5–1.0 mM) or DMSO vehicle (0.1%) for 2 h. Subsequently, tumor necrosis factor-alpha (TNF-α, 10 ng/mL) or PBS was added and cultures were further incubated for 4 h. Cells were then solubilized and lysates were resolved by 10% SDS-PAGE as described previously [ ] using Functional Assay:Article Title: Cell-in-cell structures formed between human cancer cell lines and the cytotoxic regulatory T-cell line HOZOT. Article Snippet: NHEK (normal human epidermal keratinocyte, KK-4001, Kurabo) were maintained in EpiLife–KG2 medium (Kurabo). .. The following antibodies and reagents were used for functional blocking and immunofluorescent microscopy: anti-human HLA-A,B,C (clone: W6/32, BioLegend, San Diego, CA, USA), antihuman CD2 (clone: SFC13Pt2H9) and anti-human CD62L (clone: SFC128T17G6, Beckman Coulter, Fullerton, CA, USA), anti-human CD8 (clone: RPA-T8, BD Biosciences, San Jose, CA, USA), antisialyl Lewis A (clone: 2D3, Seikagaku Corp., Tokyo, Japan), anti-b-catenin (clone: 14, Transduction Laboratories, Lexington, KY, USA), Blocking Assay:Article Title: Cell-in-cell structures formed between human cancer cell lines and the cytotoxic regulatory T-cell line HOZOT. Article Snippet: NHEK (normal human epidermal keratinocyte, KK-4001, Kurabo) were maintained in EpiLife–KG2 medium (Kurabo). .. The following antibodies and reagents were used for functional blocking and immunofluorescent microscopy: anti-human HLA-A,B,C (clone: W6/32, BioLegend, San Diego, CA, USA), antihuman CD2 (clone: SFC13Pt2H9) and anti-human CD62L (clone: SFC128T17G6, Beckman Coulter, Fullerton, CA, USA), anti-human CD8 (clone: RPA-T8, BD Biosciences, San Jose, CA, USA), antisialyl Lewis A (clone: 2D3, Seikagaku Corp., Tokyo, Japan), anti-b-catenin (clone: 14, Transduction Laboratories, Lexington, KY, USA), Microscopy:Article Title: Cell-in-cell structures formed between human cancer cell lines and the cytotoxic regulatory T-cell line HOZOT. Article Snippet: NHEK (normal human epidermal keratinocyte, KK-4001, Kurabo) were maintained in EpiLife–KG2 medium (Kurabo). .. The following antibodies and reagents were used for functional blocking and immunofluorescent microscopy: anti-human HLA-A,B,C (clone: W6/32, BioLegend, San Diego, CA, USA), antihuman CD2 (clone: SFC13Pt2H9) and anti-human CD62L (clone: SFC128T17G6, Beckman Coulter, Fullerton, CA, USA), anti-human CD8 (clone: RPA-T8, BD Biosciences, San Jose, CA, USA), antisialyl Lewis A (clone: 2D3, Seikagaku Corp., Tokyo, Japan), anti-b-catenin (clone: 14, Transduction Laboratories, Lexington, KY, USA), Recombinase Polymerase Amplification:Article Title: Cell-in-cell structures formed between human cancer cell lines and the cytotoxic regulatory T-cell line HOZOT. Article Snippet: NHEK (normal human epidermal keratinocyte, KK-4001, Kurabo) were maintained in EpiLife–KG2 medium (Kurabo). .. The following antibodies and reagents were used for functional blocking and immunofluorescent microscopy: anti-human HLA-A,B,C (clone: W6/32, BioLegend, San Diego, CA, USA), antihuman CD2 (clone: SFC13Pt2H9) and anti-human CD62L (clone: SFC128T17G6, Beckman Coulter, Fullerton, CA, USA), anti-human CD8 (clone: RPA-T8, BD Biosciences, San Jose, CA, USA), antisialyl Lewis A (clone: 2D3, Seikagaku Corp., Tokyo, Japan), anti-b-catenin (clone: 14, Transduction Laboratories, Lexington, KY, USA), Transduction:Article Title: Cell-in-cell structures formed between human cancer cell lines and the cytotoxic regulatory T-cell line HOZOT. Article Snippet: NHEK (normal human epidermal keratinocyte, KK-4001, Kurabo) were maintained in EpiLife–KG2 medium (Kurabo). .. The following antibodies and reagents were used for functional blocking and immunofluorescent microscopy: anti-human HLA-A,B,C (clone: W6/32, BioLegend, San Diego, CA, USA), antihuman CD2 (clone: SFC13Pt2H9) and anti-human CD62L (clone: SFC128T17G6, Beckman Coulter, Fullerton, CA, USA), anti-human CD8 (clone: RPA-T8, BD Biosciences, San Jose, CA, USA), antisialyl Lewis A (clone: 2D3, Seikagaku Corp., Tokyo, Japan), anti-b-catenin (clone: 14, Transduction Laboratories, Lexington, KY, USA), Control:Article Title: Cell-in-cell structures formed between human cancer cell lines and the cytotoxic regulatory T-cell line HOZOT. Article Snippet: NHEK (normal human epidermal keratinocyte, KK-4001, Kurabo) were maintained in EpiLife–KG2 medium (Kurabo). .. The following antibodies and reagents were used for functional blocking and immunofluorescent microscopy: anti-human HLA-A,B,C (clone: W6/32, BioLegend, San Diego, CA, USA), antihuman CD2 (clone: SFC13Pt2H9) and anti-human CD62L (clone: SFC128T17G6, Beckman Coulter, Fullerton, CA, USA), anti-human CD8 (clone: RPA-T8, BD Biosciences, San Jose, CA, USA), antisialyl Lewis A (clone: 2D3, Seikagaku Corp., Tokyo, Japan), anti-b-catenin (clone: 14, Transduction Laboratories, Lexington, KY, USA), Article Title: VEGF suppresses T-lymphocyte infiltration in the tumor microenvironment through inhibition of NF-κB-induced endothelial activation. Article Snippet: Antiangiogenic treatment targeting the vascular endothelial growth factor (VEGF) signaling pathway is in clinical use, but its effect on vascular function and the tumor microenvironment is poorly understood.. Here, we investigate cross-talk between VEGF and proinflammatory TNF-a signaling in endothelial cells and its impact on leukocyte recruitment.We found that cotreatment with VEGF decreased TNF-ainduced Jurkat cell adhesion to human microvascular endothelial cells by 40%.. This was associated with inhibition of TNF-a-mediated regulation of 86 genes, including 2 T-lymphocyte-attracting chemokines, CXCL10 and CXCL11 [TNF-a concentration 1 ng/ml; 50% inhibition/inhibitory concentration (IC50) VEGF, 3 ng/ml]. Clone Assay:Article Title: CD36 Recruits α 5 β 1 Integrin to Promote Cytoadherence of P. falciparum -Infected Erythrocytes Article Snippet: Endothelial basal medium (EBM) was purchased from Lonza Walkersville, Inc. (Walkersville, MD). .. The following mAb were used: anti-human CD36 clone FA6-152 (Beckman Coulter Canada, Inc., Mississauga, ON); anti-human integrin β 1 clones TDM 29 and TS2/16 (Millipore); FITC- and PE-labelled anti-human integrin β 1 clone MEM-101A (Abcam, Cambridge, MA); anti-human α 5 clones JBS5 (Millipore); FACS:Article Title: VEGF suppresses T-lymphocyte infiltration in the tumor microenvironment through inhibition of NF-κB-induced endothelial activation. Article Snippet: Antiangiogenic treatment targeting the vascular endothelial growth factor (VEGF) signaling pathway is in clinical use, but its effect on vascular function and the tumor microenvironment is poorly understood.. Here, we investigate cross-talk between VEGF and proinflammatory TNF-a signaling in endothelial cells and its impact on leukocyte recruitment.We found that cotreatment with VEGF decreased TNF-ainduced Jurkat cell adhesion to human microvascular endothelial cells by 40%.. This was associated with inhibition of TNF-a-mediated regulation of 86 genes, including 2 T-lymphocyte-attracting chemokines, CXCL10 and CXCL11 [TNF-a concentration 1 ng/ml; 50% inhibition/inhibitory concentration (IC50) VEGF, 3 ng/ml]. |
