Review



mouse anti human icam  (R&D Systems)


Bioz Verified Symbol R&D Systems is a verified supplier
Bioz Manufacturer Symbol R&D Systems manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 93

    Structured Review

    R&D Systems mouse anti human icam
    Mouse Anti Human Icam, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 110 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+human+icam+1/Human+ICAM-1%2FCD54+Antibody/pm41944190-237-44-47
    Average 93 stars, based on 110 article reviews
    mouse anti human icam - by Bioz Stars, 2026-10
    93/100 stars

    Images

    Related Articles

    Immunostaining:

    Article Title: Inflamed lymphatic endothelium suppresses dendritic cell maturation and function via Mac–/ICAM-1-dependent mechanism
    Article Snippet: Mouse anti-human podoplanin used for FACS was from AngioBio (DelMar, CA). .. Abs used for immunostaining were: rat anti-mouse ICAM-1 (SouthernBiotech, Birmingham, AL), anti-human ICAM-1, VCAM and E-selectin (R&D Systems, Mineapolis, MN); ICAM-2 (Biosource, Camarillo, CA); mouse anti-α-smooth muscle actin FITC-conjugated (Sigma-Aldrich, St.Louis, MO) ; rabbit anti-human LYVE-1 and podoplanin (Fitzgerald Industries International, Concord, MA). .. Mouse anti-human antibodies used in blocking studies were: anti-ICAM-1, clone P2A4 (Chemicon, Temecula, CA) and clone 15.2 from Santa Cruz Biotechnology (Santa Cruz, CA); anti-LFA-1, clone TS1/22, (Pierce Biotechnology, Rockford, IL) and anti-Mac-1, clone CBRM1/5 (eBioscience, San Diego, CA).

    Incubation:

    Article Title: Mastic Oil Inhibits the Metastatic Phenotype of Mouse Lung Adenocarcinoma Cells
    Article Snippet: .. EC were treated with medium containing various concentrations of mastic oil (0.01–0.04% v/v), POH (0.5–1.0 mM) or DMSO vehicle (0.1%) for 2 h. Subsequently, tumor necrosis factor-alpha (TNF-α, 10 ng/mL) or PBS was added and cultures were further incubated for 4 h. Cells were then solubilized and lysates were resolved by 10% SDS-PAGE as described previously [ ] using anti-human ICAM-1, VCAM-1 (R&D Systems, Minneapolis, MN, USA) or anti-beta-actin (Chemicon, Temecula, CA, USA) antibodies. ..

    Article Title: VEGF suppresses T-lymphocyte infiltration in the tumor microenvironment through inhibition of NF-κB-induced endothelial activation.
    Article Snippet: Antiangiogenic treatment targeting the vascular endothelial growth factor (VEGF) signaling pathway is in clinical use, but its effect on vascular function and the tumor microenvironment is poorly understood.. Here, we investigate cross-talk between VEGF and proinflammatory TNF-a signaling in endothelial cells and its impact on leukocyte recruitment.We found that cotreatment with VEGF decreased TNF-ainduced Jurkat cell adhesion to human microvascular endothelial cells by 40%.. This was associated with inhibition of TNF-a-mediated regulation of 86 genes, including 2 T-lymphocyte-attracting chemokines, CXCL10 and CXCL11 [TNF-a concentration 1 ng/ml; 50% inhibition/inhibitory concentration (IC50) VEGF, 3 ng/ml].

    SDS Page:

    Article Title: Mastic Oil Inhibits the Metastatic Phenotype of Mouse Lung Adenocarcinoma Cells
    Article Snippet: .. EC were treated with medium containing various concentrations of mastic oil (0.01–0.04% v/v), POH (0.5–1.0 mM) or DMSO vehicle (0.1%) for 2 h. Subsequently, tumor necrosis factor-alpha (TNF-α, 10 ng/mL) or PBS was added and cultures were further incubated for 4 h. Cells were then solubilized and lysates were resolved by 10% SDS-PAGE as described previously [ ] using anti-human ICAM-1, VCAM-1 (R&D Systems, Minneapolis, MN, USA) or anti-beta-actin (Chemicon, Temecula, CA, USA) antibodies. ..

    Functional Assay:

    Article Title: Cell-in-cell structures formed between human cancer cell lines and the cytotoxic regulatory T-cell line HOZOT.
    Article Snippet: NHEK (normal human epidermal keratinocyte, KK-4001, Kurabo) were maintained in EpiLife–KG2 medium (Kurabo). .. The following antibodies and reagents were used for functional blocking and immunofluorescent microscopy: anti-human HLA-A,B,C (clone: W6/32, BioLegend, San Diego, CA, USA), antihuman CD2 (clone: SFC13Pt2H9) and anti-human CD62L (clone: SFC128T17G6, Beckman Coulter, Fullerton, CA, USA), anti-human CD8 (clone: RPA-T8, BD Biosciences, San Jose, CA, USA), antisialyl Lewis A (clone: 2D3, Seikagaku Corp., Tokyo, Japan), anti-b-catenin (clone: 14, Transduction Laboratories, Lexington, KY, USA), anti-human ICAM-1 (clone: BBIG-I1), anti-human RANTES (clone: 21445), anti-human CD31 (clone: UCHT1), antihuman CD28 (clone: 37407.111, R&D Systems Inc., Minneapolis, MN, USA), anti-human granzyme B (clone: GrB-7, Kamiya Biomedical Co., Seattle, WA, USA), anti-human perforin (clone: deltaG9, Ancell Co., Bayport, MN, USA), anti-cleaved caspase-3 (Calbiochem, Darmstadt, Germany), anti-Fas (clone: CH-11, Medical & Biological Laboratories, Nagoya, Japan), mouse monoclonal isotype control (clone: MOPC-21, Abcam, Inc., Cambridge, MA, USA), Alexa Fluor 488 goat anti-mouse IgG, Alexa Fluor 488 goat anti-rabbit IgG, CellTracker (CMFDA, CMTPX, Invitrogen, Paisley, UK), cytochalasin E (EMD Chemicals, Inc., Darmstadt, Germany) and caspase-3 inhibitor (Ac-DMQD-CHO, Calbiochem, La Jolla, CA, USA). ..

    Blocking Assay:

    Article Title: Cell-in-cell structures formed between human cancer cell lines and the cytotoxic regulatory T-cell line HOZOT.
    Article Snippet: NHEK (normal human epidermal keratinocyte, KK-4001, Kurabo) were maintained in EpiLife–KG2 medium (Kurabo). .. The following antibodies and reagents were used for functional blocking and immunofluorescent microscopy: anti-human HLA-A,B,C (clone: W6/32, BioLegend, San Diego, CA, USA), antihuman CD2 (clone: SFC13Pt2H9) and anti-human CD62L (clone: SFC128T17G6, Beckman Coulter, Fullerton, CA, USA), anti-human CD8 (clone: RPA-T8, BD Biosciences, San Jose, CA, USA), antisialyl Lewis A (clone: 2D3, Seikagaku Corp., Tokyo, Japan), anti-b-catenin (clone: 14, Transduction Laboratories, Lexington, KY, USA), anti-human ICAM-1 (clone: BBIG-I1), anti-human RANTES (clone: 21445), anti-human CD31 (clone: UCHT1), antihuman CD28 (clone: 37407.111, R&D Systems Inc., Minneapolis, MN, USA), anti-human granzyme B (clone: GrB-7, Kamiya Biomedical Co., Seattle, WA, USA), anti-human perforin (clone: deltaG9, Ancell Co., Bayport, MN, USA), anti-cleaved caspase-3 (Calbiochem, Darmstadt, Germany), anti-Fas (clone: CH-11, Medical & Biological Laboratories, Nagoya, Japan), mouse monoclonal isotype control (clone: MOPC-21, Abcam, Inc., Cambridge, MA, USA), Alexa Fluor 488 goat anti-mouse IgG, Alexa Fluor 488 goat anti-rabbit IgG, CellTracker (CMFDA, CMTPX, Invitrogen, Paisley, UK), cytochalasin E (EMD Chemicals, Inc., Darmstadt, Germany) and caspase-3 inhibitor (Ac-DMQD-CHO, Calbiochem, La Jolla, CA, USA). ..

    Microscopy:

    Article Title: Cell-in-cell structures formed between human cancer cell lines and the cytotoxic regulatory T-cell line HOZOT.
    Article Snippet: NHEK (normal human epidermal keratinocyte, KK-4001, Kurabo) were maintained in EpiLife–KG2 medium (Kurabo). .. The following antibodies and reagents were used for functional blocking and immunofluorescent microscopy: anti-human HLA-A,B,C (clone: W6/32, BioLegend, San Diego, CA, USA), antihuman CD2 (clone: SFC13Pt2H9) and anti-human CD62L (clone: SFC128T17G6, Beckman Coulter, Fullerton, CA, USA), anti-human CD8 (clone: RPA-T8, BD Biosciences, San Jose, CA, USA), antisialyl Lewis A (clone: 2D3, Seikagaku Corp., Tokyo, Japan), anti-b-catenin (clone: 14, Transduction Laboratories, Lexington, KY, USA), anti-human ICAM-1 (clone: BBIG-I1), anti-human RANTES (clone: 21445), anti-human CD31 (clone: UCHT1), antihuman CD28 (clone: 37407.111, R&D Systems Inc., Minneapolis, MN, USA), anti-human granzyme B (clone: GrB-7, Kamiya Biomedical Co., Seattle, WA, USA), anti-human perforin (clone: deltaG9, Ancell Co., Bayport, MN, USA), anti-cleaved caspase-3 (Calbiochem, Darmstadt, Germany), anti-Fas (clone: CH-11, Medical & Biological Laboratories, Nagoya, Japan), mouse monoclonal isotype control (clone: MOPC-21, Abcam, Inc., Cambridge, MA, USA), Alexa Fluor 488 goat anti-mouse IgG, Alexa Fluor 488 goat anti-rabbit IgG, CellTracker (CMFDA, CMTPX, Invitrogen, Paisley, UK), cytochalasin E (EMD Chemicals, Inc., Darmstadt, Germany) and caspase-3 inhibitor (Ac-DMQD-CHO, Calbiochem, La Jolla, CA, USA). ..

    Recombinase Polymerase Amplification:

    Article Title: Cell-in-cell structures formed between human cancer cell lines and the cytotoxic regulatory T-cell line HOZOT.
    Article Snippet: NHEK (normal human epidermal keratinocyte, KK-4001, Kurabo) were maintained in EpiLife–KG2 medium (Kurabo). .. The following antibodies and reagents were used for functional blocking and immunofluorescent microscopy: anti-human HLA-A,B,C (clone: W6/32, BioLegend, San Diego, CA, USA), antihuman CD2 (clone: SFC13Pt2H9) and anti-human CD62L (clone: SFC128T17G6, Beckman Coulter, Fullerton, CA, USA), anti-human CD8 (clone: RPA-T8, BD Biosciences, San Jose, CA, USA), antisialyl Lewis A (clone: 2D3, Seikagaku Corp., Tokyo, Japan), anti-b-catenin (clone: 14, Transduction Laboratories, Lexington, KY, USA), anti-human ICAM-1 (clone: BBIG-I1), anti-human RANTES (clone: 21445), anti-human CD31 (clone: UCHT1), antihuman CD28 (clone: 37407.111, R&D Systems Inc., Minneapolis, MN, USA), anti-human granzyme B (clone: GrB-7, Kamiya Biomedical Co., Seattle, WA, USA), anti-human perforin (clone: deltaG9, Ancell Co., Bayport, MN, USA), anti-cleaved caspase-3 (Calbiochem, Darmstadt, Germany), anti-Fas (clone: CH-11, Medical & Biological Laboratories, Nagoya, Japan), mouse monoclonal isotype control (clone: MOPC-21, Abcam, Inc., Cambridge, MA, USA), Alexa Fluor 488 goat anti-mouse IgG, Alexa Fluor 488 goat anti-rabbit IgG, CellTracker (CMFDA, CMTPX, Invitrogen, Paisley, UK), cytochalasin E (EMD Chemicals, Inc., Darmstadt, Germany) and caspase-3 inhibitor (Ac-DMQD-CHO, Calbiochem, La Jolla, CA, USA). ..

    Transduction:

    Article Title: Cell-in-cell structures formed between human cancer cell lines and the cytotoxic regulatory T-cell line HOZOT.
    Article Snippet: NHEK (normal human epidermal keratinocyte, KK-4001, Kurabo) were maintained in EpiLife–KG2 medium (Kurabo). .. The following antibodies and reagents were used for functional blocking and immunofluorescent microscopy: anti-human HLA-A,B,C (clone: W6/32, BioLegend, San Diego, CA, USA), antihuman CD2 (clone: SFC13Pt2H9) and anti-human CD62L (clone: SFC128T17G6, Beckman Coulter, Fullerton, CA, USA), anti-human CD8 (clone: RPA-T8, BD Biosciences, San Jose, CA, USA), antisialyl Lewis A (clone: 2D3, Seikagaku Corp., Tokyo, Japan), anti-b-catenin (clone: 14, Transduction Laboratories, Lexington, KY, USA), anti-human ICAM-1 (clone: BBIG-I1), anti-human RANTES (clone: 21445), anti-human CD31 (clone: UCHT1), antihuman CD28 (clone: 37407.111, R&D Systems Inc., Minneapolis, MN, USA), anti-human granzyme B (clone: GrB-7, Kamiya Biomedical Co., Seattle, WA, USA), anti-human perforin (clone: deltaG9, Ancell Co., Bayport, MN, USA), anti-cleaved caspase-3 (Calbiochem, Darmstadt, Germany), anti-Fas (clone: CH-11, Medical & Biological Laboratories, Nagoya, Japan), mouse monoclonal isotype control (clone: MOPC-21, Abcam, Inc., Cambridge, MA, USA), Alexa Fluor 488 goat anti-mouse IgG, Alexa Fluor 488 goat anti-rabbit IgG, CellTracker (CMFDA, CMTPX, Invitrogen, Paisley, UK), cytochalasin E (EMD Chemicals, Inc., Darmstadt, Germany) and caspase-3 inhibitor (Ac-DMQD-CHO, Calbiochem, La Jolla, CA, USA). ..

    Control:

    Article Title: Cell-in-cell structures formed between human cancer cell lines and the cytotoxic regulatory T-cell line HOZOT.
    Article Snippet: NHEK (normal human epidermal keratinocyte, KK-4001, Kurabo) were maintained in EpiLife–KG2 medium (Kurabo). .. The following antibodies and reagents were used for functional blocking and immunofluorescent microscopy: anti-human HLA-A,B,C (clone: W6/32, BioLegend, San Diego, CA, USA), antihuman CD2 (clone: SFC13Pt2H9) and anti-human CD62L (clone: SFC128T17G6, Beckman Coulter, Fullerton, CA, USA), anti-human CD8 (clone: RPA-T8, BD Biosciences, San Jose, CA, USA), antisialyl Lewis A (clone: 2D3, Seikagaku Corp., Tokyo, Japan), anti-b-catenin (clone: 14, Transduction Laboratories, Lexington, KY, USA), anti-human ICAM-1 (clone: BBIG-I1), anti-human RANTES (clone: 21445), anti-human CD31 (clone: UCHT1), antihuman CD28 (clone: 37407.111, R&D Systems Inc., Minneapolis, MN, USA), anti-human granzyme B (clone: GrB-7, Kamiya Biomedical Co., Seattle, WA, USA), anti-human perforin (clone: deltaG9, Ancell Co., Bayport, MN, USA), anti-cleaved caspase-3 (Calbiochem, Darmstadt, Germany), anti-Fas (clone: CH-11, Medical & Biological Laboratories, Nagoya, Japan), mouse monoclonal isotype control (clone: MOPC-21, Abcam, Inc., Cambridge, MA, USA), Alexa Fluor 488 goat anti-mouse IgG, Alexa Fluor 488 goat anti-rabbit IgG, CellTracker (CMFDA, CMTPX, Invitrogen, Paisley, UK), cytochalasin E (EMD Chemicals, Inc., Darmstadt, Germany) and caspase-3 inhibitor (Ac-DMQD-CHO, Calbiochem, La Jolla, CA, USA). ..

    Article Title: VEGF suppresses T-lymphocyte infiltration in the tumor microenvironment through inhibition of NF-κB-induced endothelial activation.
    Article Snippet: Antiangiogenic treatment targeting the vascular endothelial growth factor (VEGF) signaling pathway is in clinical use, but its effect on vascular function and the tumor microenvironment is poorly understood.. Here, we investigate cross-talk between VEGF and proinflammatory TNF-a signaling in endothelial cells and its impact on leukocyte recruitment.We found that cotreatment with VEGF decreased TNF-ainduced Jurkat cell adhesion to human microvascular endothelial cells by 40%.. This was associated with inhibition of TNF-a-mediated regulation of 86 genes, including 2 T-lymphocyte-attracting chemokines, CXCL10 and CXCL11 [TNF-a concentration 1 ng/ml; 50% inhibition/inhibitory concentration (IC50) VEGF, 3 ng/ml].

    Clone Assay:

    Article Title: CD36 Recruits α 5 β 1 Integrin to Promote Cytoadherence of P. falciparum -Infected Erythrocytes
    Article Snippet: Endothelial basal medium (EBM) was purchased from Lonza Walkersville, Inc. (Walkersville, MD). .. The following mAb were used: anti-human CD36 clone FA6-152 (Beckman Coulter Canada, Inc., Mississauga, ON); anti-human integrin β 1 clones TDM 29 and TS2/16 (Millipore); FITC- and PE-labelled anti-human integrin β 1 clone MEM-101A (Abcam, Cambridge, MA); anti-human α 5 clones JBS5 (Millipore); anti-human ICAM-1 clone 84H10 (R&D Systems, Inc Minneapolis, MN); anti-human α v β 3 clone 23C6 (Chemicon International); mouse IgG1 clone 11711 (R&D Systems); anti-phospho-Tyr418Src (BioSource; Invitrogen), anti-His-tag (His-probe (H-15)) (Santa Cruz Biotechnology Inc., Santa Cruz, CA); FITC goat anti-mouse IgG 1 (Becton Dickinson, San Diego, CA); and Alexa Fluor 488 or 568 goat anti-mouse IgG 1 antibodies and rhodamine-phalloidin (Molecular Probes, Invitrogen). .. Horseradish peroxidase (HRP)-conjugated secondary antibodies were purchased from Jackson ImmunoResearch Laboratories (West Grove, PA).

    FACS:

    Article Title: VEGF suppresses T-lymphocyte infiltration in the tumor microenvironment through inhibition of NF-κB-induced endothelial activation.
    Article Snippet: Antiangiogenic treatment targeting the vascular endothelial growth factor (VEGF) signaling pathway is in clinical use, but its effect on vascular function and the tumor microenvironment is poorly understood.. Here, we investigate cross-talk between VEGF and proinflammatory TNF-a signaling in endothelial cells and its impact on leukocyte recruitment.We found that cotreatment with VEGF decreased TNF-ainduced Jurkat cell adhesion to human microvascular endothelial cells by 40%.. This was associated with inhibition of TNF-a-mediated regulation of 86 genes, including 2 T-lymphocyte-attracting chemokines, CXCL10 and CXCL11 [TNF-a concentration 1 ng/ml; 50% inhibition/inhibitory concentration (IC50) VEGF, 3 ng/ml].



    Similar Products

    94
    Miltenyi Biotec cd54
    Cd54, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+human+icam+1/CD54+(ICAM-1)+Antibody%2C+anti-human%2C+REAfinity/pm41906686-99-14-23
    Average 94 stars, based on 1 article reviews
    cd54 - by Bioz Stars, 2026-10
    94/100 stars
      Buy from Supplier

    93
    R&D Systems mouse anti human icam
    Mouse Anti Human Icam, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+human+icam+1/Human+ICAM-1%2FCD54+Antibody/pm41944190-237-44-47
    Average 93 stars, based on 1 article reviews
    mouse anti human icam - by Bioz Stars, 2026-10
    93/100 stars
      Buy from Supplier

    93
    R&D Systems anti icam1 antibodies
    Anti Icam1 Antibodies, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+human+icam+1/Human+ICAM-1%2FCD54+Antibody/10__1016_slash_j__omtn__2026__102932-180-28-33
    Average 93 stars, based on 1 article reviews
    anti icam1 antibodies - by Bioz Stars, 2026-10
    93/100 stars
      Buy from Supplier

    94
    Miltenyi Biotec anti cd54 apc
    Anti Cd54 Apc, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+human+icam+1/CD54+(ICAM-1)+Antibody%2C+anti-human%2C+REAfinity/pm41864987-137-11-16
    Average 94 stars, based on 1 article reviews
    anti cd54 apc - by Bioz Stars, 2026-10
    94/100 stars
      Buy from Supplier

    93

    93
      Buy from Supplier

    Image Search Results


    IL-6 increases ICAM-1 expression with no bacteria present, but ICAM-1 expression may decrease in presence of high exogenous IL-6 levels and P. aeruginosa . Representative maximum intensity projections of confocal images of HUVECs seeded in microfluidic devices and incubated with 0, 10, and 100 ng/mL IL-6 for 2 hours (A) with no bacteria and (C) with P. aeruginosa . Cells were fixed and stained with Hoechst (nuclei, blue), phalloidin (actin, red), and anti-ICAM-1 (neutrophil tight binding to endothelial cells, green) (scale bar = 250 μm). Images were thresholded in ImageJ to visualize the differences in ICAM-1. Representative raw images used for protein expression analysis can be found in <xref ref-type=Supplementary Figure 3 . (B, D) Fold change of ICAM-1 expression was calculated by dividing the total fluorescence intensity of un-thresholded images for each condition by the total fluorescence intensity for the 0 ng/mL IL-6 condition of the corresponding biological replicate. Fold change values for each biological replicate were averaged together and then all IL-6 conditions were compared to each other at each time point. Conditions at each time point were compared using a one-way ANOVA and then p-values were determined using Tukey’s HSD post-hoc test. Error bars indicate the mean ± SEM. (B) Data quantified from 9 devices for 0 ng/mL, 8 devices for 10 ng/mL, and 7 devices for 100 ng/mL from 4 unique HUVEC flask replicates for protein expression when not exposed to bacteria. (D) Data quantified from 8 devices for 0 ng/mL, 9 devices for 10 ng/mL, and 8 devices for 100 ng/mL from 3 unique HUVEC flask replicates across 3 independent experiments for protein expression when exposed to P. aeruginosa . " width="100%" height="100%">

    Journal: Frontiers in Immunology

    Article Title: Interleukin-6 regulates the neutrophil response to diverse bacteria

    doi: 10.3389/fimmu.2026.1783843

    Figure Lengend Snippet: IL-6 increases ICAM-1 expression with no bacteria present, but ICAM-1 expression may decrease in presence of high exogenous IL-6 levels and P. aeruginosa . Representative maximum intensity projections of confocal images of HUVECs seeded in microfluidic devices and incubated with 0, 10, and 100 ng/mL IL-6 for 2 hours (A) with no bacteria and (C) with P. aeruginosa . Cells were fixed and stained with Hoechst (nuclei, blue), phalloidin (actin, red), and anti-ICAM-1 (neutrophil tight binding to endothelial cells, green) (scale bar = 250 μm). Images were thresholded in ImageJ to visualize the differences in ICAM-1. Representative raw images used for protein expression analysis can be found in Supplementary Figure 3 . (B, D) Fold change of ICAM-1 expression was calculated by dividing the total fluorescence intensity of un-thresholded images for each condition by the total fluorescence intensity for the 0 ng/mL IL-6 condition of the corresponding biological replicate. Fold change values for each biological replicate were averaged together and then all IL-6 conditions were compared to each other at each time point. Conditions at each time point were compared using a one-way ANOVA and then p-values were determined using Tukey’s HSD post-hoc test. Error bars indicate the mean ± SEM. (B) Data quantified from 9 devices for 0 ng/mL, 8 devices for 10 ng/mL, and 7 devices for 100 ng/mL from 4 unique HUVEC flask replicates for protein expression when not exposed to bacteria. (D) Data quantified from 8 devices for 0 ng/mL, 9 devices for 10 ng/mL, and 8 devices for 100 ng/mL from 3 unique HUVEC flask replicates across 3 independent experiments for protein expression when exposed to P. aeruginosa .

    Article Snippet: Next, a staining solution of 0.005 mg/mL Hoechst 34580 ( H21486 , Invitrogen), 5:600 anti-ICAM-1 (FITC conjugated, BBA20, R&D Systems), 1:1000 phalloidin (iFluor-555 conjugated, ab176756, Abcam), and 5:600 anti-VE-Cadherin (Alexa Fluor 647 conjugated, 561567, BD Pharmingen) was added 3 times to each device.

    Techniques: Expressing, Bacteria, Incubation, Staining, Binding Assay, Fluorescence